|
|
Indicatours of sustainable development social pillar at the local level
Kučerová, Zita ; Kyselka, Mojmír (referee) ; Doc.PhDr.Jana Šafránková,CSc. (referee) ; Staňková, Eva (referee) ; Koutný, Jan (advisor)
The aim of the thesis is to find the answer, whether it is plausible to monitor social cohesion of the municipality and how to measure this cohesion, which is one of the essential pillars of sustainable development. In order to find out, analysis of approaches and measurements of the sustainable development social pillar in international and Czech environment was carried out. Social pillar was identified and described also at the local level. Within the framework of the sustainable development social pillar at the municipal level three main thematic blocks are proposed: • Social environment quality of the municipality • Social-demographic population structure of the municipality • Contentment and participation in the public affairs These themes should be monitored by the set of indicators proposed in this thesis. It aimed to cover all dimensions of social cohesion which are significant and well-founded in the context of the territorial planning process. These indicators can be followed in the direct relationship with the population size and regional importance of the observed territorial unit at the local level and they are not separated from the concept of indicators monitoring at other – territorially higher – levels. Outcomes, analysis and interpretation of attained values could become the basis for the “territorial sustainable development analysis” (part of territorial planning instruments according to contemporary legal regulations), representing social pillar. All phenomenon and processes must be evaluated in the context with other sustainable development pillars and afterwards they should become another suitable instrument for measurement of the fulfilling the territorial planning aims.
|
|
|
Automation of genotype data calculations
Vybíhal, Adam ; Richter, Miloslav (referee) ; Štohl, Radek (advisor)
The goal of this paper is to automate work with programs for the study of population structure using genotype data. Specifically, we describe properties of four programs for this use, Structure, Structure Harvester, CLUMPP and distruct. There's proposed a process of automation of work with these programs within the paper. Based on proposed process, an application is then created on the .NET platform, which allows you to work with mentioned software. This application provides a graphical user interface for creating input files, entering the necessary parameters, setting up automatic execution of individual programs and displaying their results.
|
|
|
Automation of genotype data calculations
Janásek, Josef ; Štohl, Radek (referee) ; Richter, Miloslav (advisor)
This work is focused no creating application which automates work with applications Structure, Structure Harvester, CLUMPP a Distruct. These applications are used to work with genotype data for studying population structures. Automatization design for these purposes is then worked to an app which includes a user-friendly interface for easing the work as uploading data, filling in parameters for calculations and displaying results. Tis app is created on the platform .NET.
|
|
|
Infection pathway of Hymenoscyphus fraxineus and its interactions with ash mycobiota
Haňáčková, Zuzana ; Koukol, Ondřej (advisor) ; Adamčíková, Katarína (referee) ; Botella Sánchez, Leticia (referee)
Ash dieback is a disease that affected populations of native ash species throughout Europe at the beginning of the 21st century. It causes necrotic spots on the leaves, necrosis of shoots and branches and, especially in young individuals, the total death of the tree. In particular, F. excelsior is attacked by ash dieback. The causative agent of the disease is the invasive ascomycete fungus from Asia - Hymenoscyphus fraxineus. The aims of this dissertation thesis were as follows: 1) to study population structure of H. fraxineus at different scales - at regional scale (Czech populations) and at the level of particular ash petioles, this latter was to serve as a confirmation of the pathogen infection pathway; 2) to elucidate the effect of H. fraxineus on saprotrophs in the litter (namely H. albidus) and 3) to compare endophytic mycobiota of tolerant and susceptible F. excelsior trees and to test antagonistic interactions of these fungi against the pathogen. Analysis of Czech populations showed lower average gene diversity compared to other European populations with one Czech population differing significantly from the rest due to the lowest allelic richness and very low average gene diversity. The results indicate that despite minimal differences among European populations, local ecological or...
|
|
|
Population Structure and History of the Sahel From the Point of View of Alcohol Metabolism
Jurišová, Lívia ; Černý, Viktor (advisor) ; Macholán, Miloš (referee)
The Sahel is the northernmost part of sub-Saharan Africa. The Sahel is inhabited by a complex mixture of people, who differ in their ethnic background, language affiliation and lifestyle. The most important and most studied gene of ethanol metabolism, ADH1B, has been understudied in the Sahel region, mainly due to the low frequency of the variant rs1229984-T, which accelerates the first step of alcohol metabolism. Due to its non- African origin, the variant rs1229984-T represents a suitable marker for population history study. The eastern Sahel is where immigrants from Arabia have been settling and mating with African populations since the 7th century CE. To study population structure and history from the point of view of alcohol meta- bolism, we have used already known genotype data from fourteen Sahelian populations, which inhabit mainly the eastern part of the region. Due to the absence of the critical locus rs1229984 in the genotyping array, the samples have been subjected to sequen- cing. Combined data from the microarray and the sequencing constituted 26 loci of the ADH1B gene and its close periphery, which came from 318 samples. Genotypic data needed to be converted into their haplotypic form by a phasing prog- ram. To choose the more appropriate phasing program, the data have been arranged...
|
|
|
Phylogeography and genetic diversity of the populations of Gobius niger (Gobiiformes: Gobiidae)
Hradečný, Jakub ; Vukićová, Jasna (advisor) ; Perdices, Anabel (referee)
Gobies (Gobiiformes: Gobiidae) are the most speciose fish family and the most abundant fish group of the European seas. Yet, the population genetic diversity has been studied in only a few European goby species. Although the ocean is seemingly a continuous habitat, there are barriers to gene flow in marine environment in form of e.g. straits or marine currents. The black goby (Gobius niger) is a demersal marine fish with planktonic larvae, inhabiting the coastal waters of Europe, north Africa and the Mediterranean and Black Sea coast of the Middle East. The current work examined individuals from 28 localities across the distribution range of the species in order to study the population genetic diversity. The analyses of two mitochondrial markers (cytochrome b and D loop) revealed a complex structuring of the population and a pronounced intraspecific diversity (maximum uncorrected p-distance 6.1 %). The population is divided into four very diverse haplotype groups, with unexpected geographic pattern. While the population inhabiting the Atlantic Ocean and the North Sea is generally genetically homogeneous, with all examined individuals bearing haplotypes belonging to a single haplotype group, the Mediterranean population is very complex, displaying a presence of four haplotype groups, some of which...
|
|
| |
|
|
Application of molecular methods in population genetic studies
Šurinová, Mária
A vast range of factors shape the genetic structure of plant populations. In this thesis, I focus on two of them. The first factor, polyploidization, is a process of chromosome set multiplication through whole-genome duplication within a single species (autopolyploids) or hybridization of two different species (allopolyploids). It rapidly brings changes into genomes, allowing species to occupy distinct niches, adapt to new habitats, colonize them, or adapt to changing environment in their native range. But it comes at a certain cost - difficulties in mitosis and meiosis, changes in cellular architecture. Furthermore, after cytotype establishment, new individuals have to deal with cytotype exclusion effect, competition with parental individuals and higher nutrition requirements. In this thesis, I present the effect of polyploidization on populations of three species- Arabidopsis arenosa, Aster amellus and Festuca rubra. The second factor changing the population genetic structure presented in this thesis is the fragmentation of populations. Population fragmentation can be caused by natural or anthropogenic activities and often leads to overall reduction in population size and reduced connectivity among fragments. Restricted gene flow may threaten long-term population survival due to inbreeding...
|
|
|
Y chromosome in the mouse hybrid zone
Rubík, Pavel ; Munclinger, Pavel (advisor) ; Zemanová, Barbora (referee)
The contact zone between subspecies of house mouse Mus musculus musculus and Mus musculus domesticus is one of the most intensively studied hybrid zones. It is also due to extensive introgression of the Y chromosome of M. m. musculus subspecies to the genetic background of M. m. domesticus. One theory of the origin of the introgression explains it by intragenomic conflict between the sexes. With a set of variable microsatellite markers on the Y chromosome, I have examined the validity of this theory by simple approaches revealing the history of the introgression area. It turned out that overly big variability of our markers makes the revelation of this theory impossible. Our markers have been found suitable for use in the analysis of population structure of house mouse. Thanks to them, we can identify migrants between localities and estimate the level of closeness of the population structure in relation to migrants from the neighborhood. Populations in our analysis proved to be relatively closed and resistant to the influx of migrants. Despite the conclusions of previous research where the dispersion of males ran up to one kilometer, I have discovered a relatively large number of migrations to a distance of thirty kilometers. Keywords Mus musculus musculus, Mus musculus domesticus, Y chromosome,...
|
|
|
Population structure of the gray wolf (Canis lupus)
Říhová, Jana ; Hulva, Pavel (advisor) ; Pivnička, Karel (referee)
Gray wolf (Canis lupus), is historicaly the most widespread carnivore with the former range including most of the northern hemisphere. The ambivalent relationship of humans to this predator resulted in extermination of this species in many areas, on the other hand led to the evolution of the dog, unique domesticant with vast phenotypic variation. The effort of conservation biology in last decades resulted in the shift of perception the role of big carnivores in ecosystems, with consequent recolonization of many regions by wolf, including the Czech republic. This changes brings the elevation of interest in this taxon by both experts and laymans. Present review aims to assemble published information regarding distribution, phylogeography, population structure, social and spatial behaviour of the gray wolf. The chapters describing the domestication process of the dog and hybridization with his wild ancestor are included.
|
guest ::
